Effect of poly (ADP-ribose) polymerase inhibitor combined with carbo on apoptosis of human breast cancer cells
10.3785/j.issn.1008-9292.2015.09.06
- VernacularTitle:聚腺苷二磷酸核糖聚合酶抑制剂联合卡铂对人乳腺癌细胞凋亡的影响
- Author:
Quan-Wei TAO
1
;
Xiang-Yang XIA
;
Qun-Chao MA
;
Bo YANG
Author Information
1. 浙江大学医学院附属第二医院心内科
- Keywords:
Breast neoplasms/drug therapy;
Poly ( ADP-ribose ) polymerases/antagonists & inhibitors;
Poly ( ADP-ribose ) polymerases/pharmacology;
Carboplatin/pharmacology
- From:
Journal of Zhejiang University. Medical sciences
2015;(5):506-510
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of poly ( ADP-ribose ) polymerase ( PARP) inhibitor ABT888 combined with carbo on apoptosis of human breast cancer cells.Methods:MTT was used to detect the cell viability of MDA-MB-435s cells after treatment of carbo and ABT 888 with different concentration .FACS and Western-blotting were used to detect the cell apoptosis rate and apoptosis-related protein expression , respectively . Results: Combined application of carbo and ABT 888 significantly inhibited the proliferation of MDA-MB-435 s cells, and the inhibition rates were significantly higher than that of carbo or ABT 888 alone .The combination of carbo and ABT888 markedly induced cell apoptosis (26.3%±1.5%) more than carbo (18.6%± 1.6%, P<0.01) and ABT888 (14.7%±2.3%, P<0.01) alone.Combination of carbo and ABT888 significantly down-regulated the expression of anti-apoptosis factors Bcl-2 and up-regulated the pro-apoptosis proteins Bax and cleaved caspase-3. Conclusion:The combination of carbo and ABT 888 can suppress the proliferation and induce apoptosis of human breast cancer DA-MB-435s cells.
