Combination of lapatinib with chlorogenic acid inhibits breast cancer metastasis by suppressing macrophage M2 polarization
10.3785/j.issn.1008-9292.2015.09.04
- VernacularTitle:拉帕替尼与绿原酸联合应用抑制巨噬细胞M2型极化及抗乳腺癌转移的作用研究
- Author:
Jie-Qiong ZHANG
1
;
Zhang-Ting YAO
;
Gui-Kai LIANG
;
Xi CHEN
;
Hong-Hai WU
;
Lu JIN
;
Ling DING
Author Information
1. 浙江大学药学院浙江省抗肿瘤药物临床前研究重点实验室
- Keywords:
Key words] Breast neoplasms/drug therapy;
Neoplasm metastasis;
Deoxycytidine/analogs& derivatives;
Deoxycytidine/therapeutic use;
Antineoplastic drugs ( TCD )/therapeutic use;
Chlorogenic acid/therapeutic use;
Macrophages;
Interleukin-13
- From:
Journal of Zhejiang University. Medical sciences
2015;(5):493-499
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To determine the effect of the combination of lapatinib with chlorogenic acid on metastasis of breast cancer in mouse model . Methods: The classical macrophage M2 polarization model induced by interlukin 13 in vitro was adopted in the study .Flow cytometric analysis was performed to detect the expression of M2 marker CD206 . The transcription of M2-associated genes was measured by RT-PCR.HE staining was used to analyze the metastatic nodes of breast cancer in lungs of MMTV-PyVT mice.Immunostaining analysis was used to detect the expression of related proteins in breast cancer .Results:The combination of lapatinib and chlorogenic acid inhibited the expression of CD206 induced by IL-13 [(42.17% ±2.59%) vs ( 61 .15%±7 .58%) , P<0 .05 ] . The combination more markedly suppressed expression of M2-associated gene Ym1 than lapatinib alone [(0.9 ±0.1) vs (1.8 ± 0 .0 ) , P <0 .05 ] .The combination of lapatinib and chlorogenic acid significantly reduced metastatic nodes in lung [ P <0 .05 ] , and also significantly decreased the percentage of CD206 +cells in breast cancer compared to controls [(6.08%±2.60%) vs ( 29 .04%±5 .86%) , P<0 .05 ] .Conclusion: The combination of lapatinib and chlorogenic acid can effectively inhibit macrophage M 2 polarization and metastasis of breast cancer .
