Expression of S100A8 and A100A9 in giant cell tumor of bone and its relation with CT and MR imaging findings
10.3785/j.issn.1008-9292.2015.05.14
- VernacularTitle:骨巨细胞瘤组织 S100 A8和 S100 A9表达及与肿瘤影像学表现的相关性分析
- Author:
Jin-Sheng LIAO
1
;
Xiao-Yi DING
;
Shun-Liang XU
Author Information
1. 浙江大学医学院附属第一医院放射科
- Keywords:
Giant cell tumor of bone/pathology;
Giant cell tumor of bone/radiography;
RNA,messenger/genetics;
S100 proteins/biosynthesis;
Tomography,X-ray computed;
Magnetic resonance imagine
- From:
Journal of Zhejiang University. Medical sciences
2015;(3):329-334
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the mRNA and protein expression levels of S100A8 and S100A9 in giant cell tumor ( GCT ) of bone, and its relation with radiological findings and biological behavior .Methods:Forty three patient with GCT of bone admitted in Ruijin Hospital Shanghai Jiaotong University School of Medicine from January 2009 to June 2012 were enrolled in the study .The expression levels of S 100 A8 and S100 A9 mRNA and protein were detected by using semiquantitative RT-PCR and Western blotting in 43 specimens of GCT and 6 specimens of normal bone marrow .The CT and MRI findings of patients were retrospectively reviewed , its relation with tissue expression of S100 A8 and S100 A9 was analyzed .Results: Among 43 GCT cases 40 showed positive expression of S 100 A8 and S100 A9 mRNA and protein , and the expression levels were significantly higher than those in normal bone marrow (P<0.05).The expression level of S100A8 protein was significantly different in bone GCT with different composition ratio on MRI ( P <0 .05 ) .The expression level of S100 A9 protein was significantly different in GCT with different degree of bone destruction on CT scan ( P <0.05 ).Conclusion: The expression of S100A8 and S100A9 mRNA and protein is up-regulated in GCT of bone .The expression of S100A8 and S100 A9 is associated with the real composition ratio and the degree of bone destruction, respectively, indicating that S100A8 and S100A9 may be involved in the biological behavior of bone GCT .
