AQP5 gene silencing inhibits proliferation and migration of ectopic endometrial glandular epithelial cells in endometriosis
10.3785/j.issn.1008-9292.2015.05.08
- VernacularTitle:稳定沉默水通道蛋白5基因对异位子宫内膜腺上皮细胞增殖及迁移的影响
- Author:
Liao-Bing XIN
1
;
Xiu-Xiu JIANG
;
Xiao-Lei YE
;
Rui-Jin WU
;
Kai-Hong XU
;
Jun-Yan MA
;
Jun LIN
Author Information
1. 浙江大学医学院附属妇产科医院妇科
- Keywords:
Endometriosis;
Aquaporin 5;
Endometrium;
Gene silencing;
Epithelial cells/metabolism;
Cell proliferation;
Cell movement
- From:
Journal of Zhejiang University. Medical sciences
2015;(3):285-292
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of aquaporin 5 ( AQP5 ) on proliferation and migration of ectopic endometrial epithelial cells .Methods: AQP5 shRNA interference fragments were designed and transfected into ectopic endometrial epithelial cells stably by lentivirus technology .Fluorescence quantitative RT-PCR and Western blotting were used to detect the AQP 5 mRNA and protein expression , respectively .The cell proliferation and migration were determined by using MTT method and Transwell system , respectively .Levels of phosphorylated AKT ( p-AKT ) and total AKT were examined by Western blotting .The nude mice model of endometriosis was constructed and the endometrial cell nodule formation was observed .Results: AQP5 shRNA transfection inhibited cell proliferation and migration compared with control group(both P<0.05).The activation of AKT in AQP5 shRNA transfected cells was lower than that in control cells(P<0.01).Compared to control group, the endometrial cells nodule formation was suppressed in mice inoculated with AQP 5 shRNA-silencing ectopic endometrial epithelial cells .Conclusion: Down-regulation of AQP5 expression can suppress the proliferation and migration of ectopic endometrial epithelial cells and endometrial cell nodule formation in nude mice , in which AKT pathway may be involved.
