Expression of neuron-specific enolase and synaptophysin in esophageal development of human embryos
10.3785/j.issn.1008-9292.2015.03.011
- VernacularTitle:神经元特异性烯醇化酶和突触素在人胚胎食管组织发育阶段的表达
- Author:
Xue-Hong LIU
1
;
Yong ZHANG
;
Chuan-Kang LIU
Author Information
1. 绍兴文理学院医学院
- Keywords:
Esophagus/patholoy;
Esophagus/embryology;
Phosphopyruvate hydratase;
Synapsins;
Immunohistochemistry
- From:
Journal of Zhejiang University. Medical sciences
2015;(2):184-188
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the expression of neuron-specific enolase (NSE) and synaptophysin (SYN) proteins in different developmental stages of human embryonic esophagus . Methods: Immunohistochemistry was used to detect the expressions of NSE and SYN proteins in embryonic esophagus tissues of fetuses of 2, 3 and 4 month gestational age ( n=16 ) .One-way ANOVA and LSD-t test were employed to compare the staining intensity and number of positive expression cells in embryonic esophageal tissues of different gestational age .Results: In fetuses with 2, 3 and 4 months of gestation , the number of NSE-positive nerve cells in the myenteric nerve plexus and submucosa of human embryonic esophageal tissues were 18.38 ±8.37, 25.00 ±11.54 and 38.00 ±15.09, respectively;the staining intensity of NSE-positive nerve cells and nerve fibers in myenteric nerve plexus and submucosa of embryonic esophageal tissues were 74.38 ±14.93, 62.25 ±18.59 and 56.44 ±14.70, respectively .NSE-positive cells were detected in the esophageal epithelium only at the third month.In the fetuses at 2, 3 and 4 months of gestation , SYN in all layers of esophageal tissue were positively or strong positively expressed , especially in the myenteric plexus and submucosal plexus .The staining intensity of SYN-positive cells in embryonic esophagus tissues of 2, 3 and 4 month gestation were 54.69 ±9.34, 51 .84 ±6 .10 and 46 .41 ±6 .44 , respectively .Conclusion: SYN and NSE may be involved in the regulation of nerve system of esophageal tissues during the human embryonic development .
