Screening of active compounds with myocardial protective effects from Tongmai Yangxin pill
10.3785/j.issn.1008-9292.2015.03.005
- VernacularTitle:通脉养心丸中心肌保护活性物质的筛选
- Author:
Shan TAO
1
;
Xin-Ying LIANG
;
Yi WANG
;
Yi WANG
Author Information
1. 浙江大学药学院
- Keywords:
Myocytes,cardiac;
Anoxia;
Chromatography,high pressure liquid;
Glycyrrhiza uralensis/drug effects;
Flavones/pharmacology;
Glycyrrhizic acid/pharmacology;
Schisandra chinensis;
Ophiopogon japonicus;
Disease models,animal
- From:
Journal of Zhejiang University. Medical sciences
2015;(2):145-153
- CountryChina
- Language:Chinese
-
Abstract:
Objective: Based on cell model and HPLC-MS technology , to screen myocardial protection active compounds from traditional patent medicine Tongmai Yangxin pill ( TMYXP ) . Methods: Fractions of TMYXP were prepared by high performance liquid preparation technology . The cardioprotective effects of prepared fractions were tested on H2O2 oxidation-damaged H9c2 myocardiocytes.The active components were analyzed by high performance liquid chromatography ( HPLC) coupled with high resolution mass spectrometry .The possible active compounds were putatively identified by comparison of their MS ions and molecular weight with literatures . Results:Ten TMYXP components presented significant myocardial protective activities , 5 of which were investigated and presented good dose-effect relationships .Their median effective concentrations ( EC50 ) were respectively 11.66, 17.44, 13.10, 7.332, 15.15 μg/mL.Totally, 11 potential active compounds were analyzed and identified , including Glycyrrhizic acid , Glycycoumarin , Licoisoflavone , Ophiopogonin D′, Licoricon, Gancaonin L, Neoglycyrol, Emodin, Angeloylgomisin H, Angeloylgomisin Q and Glyasperin A .Conclusion:The myocardial protection active compounds of TMYXP were screened successfully .
