Treatment of rheumatoid arthritis with flavonoids of Echinps latifolius Tausch in rat model
10.3785/j.issn.1008-9292.2015.01.007
- VernacularTitle:禹州漏芦总黄酮对类风湿关节炎大鼠治疗机制研究
- Author:
Cheng-Gui MIAO
1
;
Guo-Liang ZHOU
;
Mei-Song QIN
;
Jian-Zhong CHEN
;
Cheng-Feng LI
;
Bing ZHANG
Author Information
1. 安徽科技学院食品药品学院
- Keywords:
RHAPONTICUM UNIFLORUM;
Flavones/pharmacology;
Arthritis,rheumatoid/pathology;
Arthritis,rheumatoid/drug therapy;
Matrix metalloproteinase 3;
bcl-2-associated X protein;
Caspase 3;
Genes,bcl-2;
Synovial membrane/cytology
- From:
Journal of Zhejiang University. Medical sciences
2015;(1):43-48
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of Flavonoids extracted from Echinps latifolius Tausch(FELT) on rheumatoid arthritis (RA) in rat model.Method:Fifty SD rats were randomly divided into model group , control group , and low, medium, and high-dose FELT groups (n=10 in each group).Complete Freund’s adjuvant (0.1 mL) was used to induce RA in rats .FELT in doses of 50 mg/kg, 100 mg/kg, 150 mg/kg was given to rats in low , medium and high-dose FELT groups by gavage , and same volume of PBS was given to rats in control group .The arthritis score and the paw swelling score were measured to evaluate the therapeutic effect of FELT .Real time qPCR was used to detect the mRNA expression of fibronectin and MMP 3 in synovial tissue and the mRNA expression of caspase 3, Bcl-2 and Bcl-2 associated X protein ( Bax) in fibroblast-like synoviocytes ( FLS) .Results:The arthritis score and the paw swelling score were significantly decreased in three FELT groups compared to RA model rats ( P<0.05 ) .The relative expression levels of FN and MMP 3 mRNA in synovium of three FELT-treatment groups were significantly lower than those in model group (1.80, 1.76 and 1.67 vs 2.53;1.69 , 1.46 and 1.45 vs 2.67 , respectively , all P<0.05 ) . The relative expression levels of Bax and caspase 3 mRNA in FLSs of three FELT groups were higher than those in model group ( 0.56 , 0.58 and 0.60 vs 0.30;0.54 , 0.56 and 0.59 vs 0.29 , respectively , all P<0.05 ); while the relative expression levels of Bcl-2 mRNA in FELT groups were lower than that in model group ( 2.20 , 2.08 and 2.08 vs 4.04 , respectively , P<0.05 ) .Conclusion:FELT may inhibit the synovium proliferation in RA model rats through promoting the FLS apoptosis .
