Protective effect of grape seed proanthocy anidin on cultured RGC-5 cells against CoCl2-induced hypoxic injury
10.3785/j.issn.1008-9292.2015.01.004
- VernacularTitle:葡萄籽原花青素对氯化钴诱导的视网膜神经节细胞 RGC－5缺氧损伤的保护作用
- Author:
Ka-Na LIN
1
;
Mei-Li LIN
;
Er-Qing WEI
Author Information
1. 浙江大学医学院附属第二医院药剂科
- Keywords:
VITIS VINIFERA/chemistry;
Proanthocyanidins/pharmacology;
Cobalt/pharmacology;
Retinal ganglion cells/drug effects;
Anoxia/therapy
- From:
Journal of Zhejiang University. Medical sciences
2015;(1):24-29
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the protective effects of grape seed proanthocyanidin extracts ( GSPE ) against CoCl 2-induced hypoxic injury in cultured RGC-5 cells.Mtehods: CoCl 2( 400 μmol L/) was used to induce hypoxic injury in cultured RGC-5 cells;the cells were pretreated with 0, 100, 200, 400 and 800μmol/L GSPE for 24h. The cell viability was assayed by MTT; the apoptosis was detected by Hoechst 33342 staining;the intracellular reactive oxygen species ( ROS) was measured by H2DCFDA oxidative reaction.The mRNA expression of Bcl-2, caspase 9 and caspase 3 was determined by real-time PCR.Results: Compared to hypoxic control group, pretreatment with GSPE significantly increased viability of RGC-5 cells ( P <0.001 ) , reduced cell apoptosis ( P<0.001 ) and intracellular ROS ( P<0.001 ) .In addition , GSPE significantly increased the mRNA expression of Bcl-2 (P<0.001 ) and decreased mRNA expression of caspase 9 ( P <0.001 ) and caspase 3 ( P<0.001 ) compared to hypoxic control group .Conclusion: GSPE may have a protective effect against CoCl 2-induced hypoxic injury in cultured RGC -5 cells. The decrease of intercellular ROS , up-regulation of Bcl-2 and down-regulation of caspase 9 and caspase 3 may be involved in the mechanism of the protective effect of GSPE .
