Efficacy of epigallocatechin gallate in treatment of alkali burn injury of murine cornea
10.3785/j.issn.1008-9292.2015.1.003
- VernacularTitle:表没食子儿茶素没食子酸酯对小鼠角膜碱烧伤的治疗作用
- Author:
Lian-Qun WU
1
;
Min LU
Author Information
1. 第二军医大学附属长征医院眼科
- Keywords:
Catechin/pharmacology;
Gallic acid/pharmacology;
Cornea/drug effects;
Alkalies;
Burns,chemical/pathology;
Corneal neovascularization;
Inflammation
- From:
Journal of Zhejiang University. Medical sciences
2015;(1):15-23
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the efficacy of epigallocatechin gallate ( EGCG) in treatment of corneal alkali burn injury in mice .Methods:Corneal alkali burn injury was induced by sodium hydroxide method in C 57BL/6J mice.The mice with cornea burns were treated intraperitoneally with EGCG solution or phosphate buffer solution ( PBS ) respectively . The healing of corneal epithelium , the formation of corneal neovascularization ( CNV ) and the inflammation reaction were assessed by slit-lamp microscopy and histological examination . Expression of vascular endothelial growth factor ( VEGF ) mRNA and protein in cornea was evaluated by real-time reverse transcription polymerase chain reaction ( RT-PCR ) and immunohistochemistry , respectively .Myeloperoxidase ( MPO ) assay was used to quantitatively evaluate the polymorphonuclear neutrophils ( PMNs ) infiltration in the corneas . Results: The healing rate of corneal epithelium in EGCG group was significantly higher than that of PBS group at d1, d3 and d7 after treatment ( d1: 41.0% ±13.0% vs 23.8% ± 7.6%;d3:76.6%±7.5% vs 61.2%±6.8%; d7: 87.8%±8.5% vs 74.0%± 9.1%;all P <0.05 ) .The CNV scores and the number of CNV in the corneal sections of EGCG group were significantly lower than those of PBS group at d 3 , d7 and d14 after treatment ( CNV score: d3: 1.1 ±0.5 vs 6.6 ±1.0; d7: 1.3 ±0.3 vs 8.1 ±1.0;d14:0.9 ±0.2 vs 9.2 ±1.1; CNV number: d3:1.68 ±0.61 vs 2.92 ±0.95; d7:4.80 ±1.36 vs 7.92 ±1.28;d14:3.64 ±0.71 vs 5.88 ±0.76;all P <0.05 ) .The expression of VEGF protein at d3 (0.19 ±0.05 vs 0.45 ±0.08) and d7 (0.42 ±0.07 vs 0.84 ±0.09 ) , the expression of VEGF mRNA at d 1 , d3 and d7 in EGCG group were significantly lower than those in PBS group ( all P <0.05 ) .Compared to PBS group, the inflammatory index at d3 (3.2 ±0.4 vs 3.7 ±0.5) and d7 (2.3 ±0.5 vs 4.0 ±0.0 ) , the number of PMNs in the corneal sections and the MPO values at d 3 , d7 and d14 in EGCG group were significantly decreased ( PMNs: d3: 34.5 ±15.7 vs 90.0 ±28.8;d7:17.1 ±11.4 vs 54.9 ±25.9;d14:12.8 ±4.6 vs 39.0 ±17.9; all P <0.05 ) .Conclusion: In the murine corneal alkali burn model , intraperitoneal injection of EGCG solution can promote the healing of corneal epithelium , inhibit the formation of CNV and reduce the inflammatory cell infiltration in the corneas .
