Screening and detection of patients with macroprolactinemia by application of polyethylene glycol precipitation method
10.3785/j.issn.1008-9292.2014.03.012
- VernacularTitle:运用聚乙二醇6000沉淀筛检巨催乳素血症患者
- Author:
Yong-Jian CHEN
1
;
Yong-Lie ZHOU
;
Li XU
;
Jie YAN
Author Information
1. 浙江大学医学院基础医学部
- Keywords:
Precipitin tests;
Polyethylene glycols/diagnostic use;
Hyperprolactinemia/diagnosis;
Prolactin;
Mass screening
- From:
Journal of Zhejiang University. Medical sciences
2014;(2):187-192
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a polyethylene glycol ( PEG6000 ) precipitation method for screening macroprolactinemia in patients with high serum prolactin ( PRL) . Methods:PEG6000 precipitation method was used to remove macroprolactin ( MPRL) molecules in serum of PRL-elevated patients.The effect of PEG6000 precipitating serum MPRL was determined by Sephacryl S-100 HR chromatography plus chemiluminescent immunoassay and SDS-PAGE plus Western Blot assay .The PEG6000 precipitation plus chemiluminescent immunoassay was applied to screen serum samples of PRL-elevated patients for macroprolactinemia .The clinical manifestations of patients with true-hyperprolactinemia , hyperprolactinemia/macroprolactinemia or true-macroprolactinemia were analyzed and compared .Results: After precipitation with PEG6000 , MPRL peak or hybridization signal in the serum samples was markedly decreased, while the big or small prolactin (BPRL or SPRL) levels were not affected. In 1538 PRL-elevated patients , 16 .1% ( 247/1538 ) were detectable for macroprolactinemia , while the 83 .9% ( 1291/1538 ) were identified as true-hyperprolactinemia .In 247 samples of macroprolactinemia , 93 .5% ( 231/247 ) were determined as true-macroprolactinemia, while 6.5% ( 16/247 ) were identified as hyperprolactinemia plus macroprolactinemia .In 508 true-hyperprolactinemia patients , menoxenia , menolipsis/menostasia , dysgenesia or hypophysoma were manifested in 438 (86.2%), which were also manifested in 85.7% ( 6/7 ) of hyperprolactinemia/macroprolactinemia patients .However , only 11 cases in 71 true-macroprolactinemia patients ( 15 .5%) presented above clinical diseases .Conclusion: There is a certain proportion of true-macroprolactinemia ( pseudo-hyperprolactinemia ) in serum PRL-elevated patients.The PEG6000 precipitation method established in this study can efficiently distinguish true-hyperprolactinemia from pseudo-hyperprolactinemia in patients .
