Purification of polyphenols from sabina vulgaris antoine and its antioxidant properties

VernacularTitle:沙地柏多酚的纯化及其抗氧化活性测定
Author: Long LI ¹ ; Gui-Da XUAN ; Ping CHEN
Author Information

1. 浙江大学城市学院医学院
Keywords: Cupressaceae/chemistry; Phenols; Antioxidants
From: Journal of Zhejiang University. Medical sciences 2014;(2):175-179
CountryChina
Language:Chinese
Abstract: Objective:To purify polyphenols from Sabina vulgaris and to investigate its antioxidant properties .Methods: Polyphenols were purified from Sabina vulgaris Antoine with macroporous resin HPD-700 , and the quantity of polyphenols was determined by Folin-Ciocalteu colorimetry .The antioxidant properties of polyphenols were evaluated by total antioxidant capacity ( T-AOC ) and its activeties of scavenging DPPH (1,1diphenyl-2-picry-hydrazyl) radicals, superoxide anion ( O2 · -), hydroxyl free radicals (OH· ) and ferric reducing antioxidant power (FRAP).Results: After purification, the purity of polyphenols increased from 0.53‰ to 9.95‰.The antioxidant properties study showed that its inhibition rate of scavenging DPPH radicals and FRAP was 151.83 U/ml and 204.59 U/ml.Its scavenging capacity for superoxide anion ( O2 · -) and hydroxyl free radicals ( OH · ) was 151.83 U/ml and 204.59 U/ml.The total antioxidant capacity was 72.68 U/ml.Conclusion:Polyphenols from Sabina vulgaris Antoine have high antioxidant properties , suggesting that it worth further study of its pharmacological effects .