Determination of R (-)-isomer in repaglinide tablets by capillary electrophoresis
10.3785/j.issn.1008-9292.2014.03.004
- VernacularTitle:毛细管电泳法检查瑞格列奈片中左旋异构体杂质
- Author:
Xiao-Wei YUAN
1
;
Jia-Yi SUN
;
Shi-Zhuo WANG
;
Xing-Jie GUO
Author Information
1. 沈阳药科大学药学院,辽宁沈阳110016
- Keywords:
Carbamates/analysis;
Carbamates/analogs & derivatives;
Electrophoresis,capillary/methods;
Stereoisomerism;
Repaglinide
- From:
Journal of Zhejiang University. Medical sciences
2014;(2):145-149
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop a capillary electrophoresis system for enantiomeric impurity test of repaglinide .Methods:An uncoated fused silica capillary ( 50 μm ×50 cm, with an effective length of 41 cm) was used.The running buffer was composed of 30 mmol/L NaH2 PO4 and 5 mg/ml carboxymethyl-β-cyclodextrin ( pH 3.5).Results: Linear range was 2.00-80.00 μg/ml (correlation coefficient was 0.9993).The average recovery rate was 92.5%to 105.0%.Conclusion:The method is simple , accurate and sensitive and it can be used for determination of enantiomeric impurities in repaglinide tablet .
