Quantitative determination of voglibose contents in its tablets with high-performance liquid chromatography-mass spectrometry
10.3785/j.issn.1008-9292.2014.03.003
- VernacularTitle:高效液相色谱-质谱法测定伏格列波糖片中伏格列波糖含量
- Author:
Xiang-Qun ZHANG
1
;
Ling-Hua WU
;
Fang-Jun CHEN
;
Yi ZHANG
;
Lu CHEN
Author Information
1. 正大青春宝药业有限公司
- Keywords:
Inositol/analysis;
Inositol/analogs & derivatives;
Retrieving rate;
Temperature;
Chromatography,high pressure liquid;
Mass spectrometry;
Voglibose
- From:
Journal of Zhejiang University. Medical sciences
2014;(2):141-144
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop a method for determination of voglibose contents in its tablets by high-performance liquid chromatography-mass spectrometry ( HPLC-MS) .Methods:The measurements were carried out on an Agilent ZORBAX Eclipse Plus C18 column (2.1 ×150mm 3.2μm) with a temperature of 40℃.A mixture of methanol and water (2∶3, v/v) was used as a mobile phase at a flow rate of 0.25 ml/min.Voglibose was detected in an electrospray ionization (ESI) mode with MRM. Results:The calibration curves of voglibose showed good linearity in a range of 1 .5804-2.6340 μg/ml (r=0.9990).The average recovery was 100.2%with RSD of 1.37%( n=6 ) for m/z 268 .2/74 .2 .Linearity was obtained with r=0 .9976 and the average recovery was 99.3%with RSD of 1.78%(n=6) for m/z 268.2/92.2.Conclusion:HPLC-MS method is accurate , reproducible and can be used for quality control of voglibose tablets .
