Quantification of Microvascular Dentsity and Cell Nuclear DNA Content in Laryngeal Cancer
10.3785/j.issn.1008-9292.2001.02.008
- VernacularTitle:喉癌微血管密度与细胞DNA含量参数的定量研究
- Author:
Yu-Yu LU
1
;
Peng SHEN
;
Li-Rong CHEN
Author Information
1. The First Affiliated Hospital College of Medical Sciences
- From:
Journal of Zhejiang University. Medical sciences
2001;30(2):73-75
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the relationship between the microvascular density,cell nuclear DNA content and the prognosis in larynge al cancer patient.Methods: In 31 cases,microvascular density was obs erved with anti-factor Ⅷ antibody and SABC method,and the proliferating nuclea r DNA content was examined with image cytometry.Results: The microva scular dencity and DNA content were not related to histologic grade and type of laryngeal cancer,but the differences of microvascular density were significant ( P＜0.05) between the patients with metastasis (10.23±0.78) and these withou t metastasis (6.38±0.33) after two years.The measurement by the image cytometry showed that the metastasis groups had a mean nuclear peak area value of (61.03 ±0.28)μm2 and the mean stage cell value of (38.11±0.37)%,and those of the g roups without metastasis were (40.97±0.41)μm2 and (22.97±0.29)%,respectivel y.There were significant differences (P＜0.05) between them.Conclusion: The angiogenesis and proliferating nuclear DNA content o f laryngeal cancer are correlated with the lymph node metastasis and prognosis.
