Construction of Prokarocyte Expression Recombinant of Human Thymosin α 1 and Its Expression in Escherichia coli
10.3785/j.issn.1008-9292.2001.02.002
- VernacularTitle:人胸腺素α1原核表达重组体的构建及其在大肠杆菌中的表达
- Author:
Feng XU
1
;
Zhi CHEN
;
Jian-Guo XU
Author Information
1. The First Affiliated Hospital College of Medical Sciences
- From:
Journal of Zhejiang University. Medical sciences
2001;30(2):52-54,58
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To acquire human thymosin α1(Tα 1) pep tides by gene engeering techniques.Methods: Tα1 gene was acquired b y molecular biological techniques and cloned into vector pGEMEX1-Tα1.An approp riate Escherichia coli (E.coli) strain was transformed by the recombinant pG EMEX1-Tα1 and its transcription and expression in E.coli we tested.Results: pUC18-Tα1,the cloning recombinant of human Tα1,was const ructed and confirmed by sequencing,and pGEMEX1-Tα1,the prokarocyte expression recombinant of human Tα1,was successfully constructed.Then it was confirmed tha t pGEMEX1-Tα1 could be transcribed in JM109 (DE3).A 36 kD fusion protein was p roduced after JM109 (DE3) transformed with pGEMEX1-Tα1 and induced with IPTG.Conclusion: The recombinant pGEMEX1-Tα1 can be successfully constr ucted and expressed in E.Coli.
