The Selection of Optimal Medium and Condition for the Production of Lac tase by Yeast Kluyvermyces fragilis
10.3785/j.issn.1008-9292.2001.02.001
- VernacularTitle:产乳糖酶酵母Kluyveromyces fragilis株培养产酶发酵条件的优化
- Author:
Qian DING
1
;
Yan-Lin JIANG
;
Jin-Yu SHAO
Author Information
1. 浙江大学
- From:
Journal of Zhejiang University. Medical sciences
2001;30(2):49-51
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the optimal condition on lactase production by yeast Kluyveromyces fragilis(K.fragilis) to increase the enzyme productivity.Methods: With separated proliferation and induction culture,the effe cts of carbon source and nitrogen source on yeast proliferation and lactase indu ction were studied by the method of monofactorial analysis.Results: The optimum medium for the productivity of lactase by K.f ragilis was determined.Maximal enzyme activity (122.4 IU/ml) was reached when the concentration of lactose was 12%,ammonium acetate was 0.5% and pH 6.7.Conclusion: The results of this experiment demonstrate that this str ain will be good for industrial production.
