Detection of Mycoplasma Pneumoniae in Children with Pneumonia

VernacularTitle:肺炎患儿肺炎支原体感染的实验室检测
Author: Xi-Lin YU ¹ ; Zhong-Sheng YU ; Tian-Lin WANG
Author Information

1. The Affiliated Children'Hospital College of Medi-cal Sciences Zhe jiang University
Keywords: Pneumoniae,mycoplasma/diag; Pneumoniae,mycoplasma/blood; Polymerase chain reaction; Mycoplasma,pneumoniae/isol; Children
From: Journal of Zhejiang University. Medical sciences 2000;29(2):67-69
CountryChina
Language:Chinese
Abstract: To evaluate the methods for detection and diagnosis of mycoplasma infections in children with pneumonia. Methods:The polymerase chain reaction (PCR) technique,mycoplasma culture and indirect haemagglutination assay (IHA) were used to detect mycoplasma pneumoniae in clinical samples obtained from 676 children (411 male and 265 female) with pneumonia. Results: Of 676patients with pneumoniae, 115 children were confirmed to be positive of mycoplasma pneumoniae. The positive rate of detection was significantly higher with PCR than with mycoplasma culture (P<0. 01 ). There was no significant difference between the positive rate of detection with PCR and that with IHA (P>0. 05). Conclusion:PCR method and IHA are highly applicable in routine clinical laboratories for the preliminary detection and diagnosis of mycoplasmal infections,particularly in extrapulmonary cases.