Expression of Intercellular Adhesion Molecule-1 in Rat Heart with Ischemia/Reperfusion Injury and Its Association with Ischemia/Reperfusion Injury
10.3785/j.issn.1008-9292.2000.02.002
- VernacularTitle:细胞间粘附分子-1在心肌缺血再灌注损伤中的表达及其相关性
- Author:
Ying ZHU
1
;
Guo-Sheng FU
;
Jiang SHAN
Author Information
1. 浙江大学附属第二医院
- Keywords:
Cell adhesion molecules/blood;
Myocardial reperfusion injury;
Animals,laboratory;
Rats
- From:
Journal of Zhejiang University. Medical sciences
2000;29(2):52-54
- CountryChina
- Language:Chinese
-
Abstract:
To study the expression of intercellular adhesion molecule-1 in rat heart with ischemia/reperfusion injury and its association with ischemia/reperfusion injury. Methods:Fourty-two male Sprague-Dawley rats were subjected to coronary artery ligation as ischemia/reperfusion heart model and were randomly divided into seven groups of six rats each. The ischemia time was 30 min.The rat hearts were excised at 0,30,60,360,540,720 min after reperfusion. The ischemic and reperfused myocardium was examined immunohistochemically to localize ICAM-1. Malondialdehyde(MDA) and nitric oxide (NO) contents in the myocardium were also determined by thiobarbituric acid and Griss method,respectively. Results: ①At the time of 540 min after reperfusion,expression of ICAM-l in rat hearts was upragulated. ②The MDA values were increased during reperfusion (P<0. 05) and reached the peak 1 hour after reperfusion. ③The NO values get lower and lower during reperfusion. Conclusion :Expession of ICAM-1 in rat hearts subjected to ischemia followed by reperfusion was increased in the late-stage of reperfusion,which might be associated with delayed reperfusion injury.
