T-lymphocyte Activation and Chemotaxis in Patients with Atopic Dermatitis
10.3785/j.issn.1008-9292.2000.02.001
- VernacularTitle:异位性皮炎患者循环T细胞激活表型和化学趋化功能研究
- Author:
Min ZHENG
1
;
Sui-Qing CAI
;
Guo-Jun SUN
Author Information
1. 浙江大学附属第二医院
- Keywords:
Dermatitis,atopic/immunol;
T-lymphocytes;
Chemotaxis;
Interleukin-8
- From:
Journal of Zhejiang University. Medical sciences
2000;29(2):49-51
- CountryChina
- Language:Chinese
-
Abstract:
In order to determine whether circulating T cells from patients with atopic dermatitis were also involved in the disease process. Methods:The authors investigated T cell activation markers and the biological behavior. A 48 microchemtaxis chamber was employed to determine T cell chemotaxis activity in 21 patients with atopic dermatitis and 20 normal controls;the expression of T cell activation markers HLA-DR and interleukin-2 receptor (IL-2R) were analysed with fluorescence activated cell sorting technique and serum interleukin-8 (IL-8) levels were measured with ELISA method. Results:T cell chemotactic responses were significantly decreased in patients with atopic dermatitis compared with healthy controls. Increased expression of HLA-DR and IL-2R were demonstrated in circulating T cells from those patients comparied with healthy controls. Serum IL-8 levels werefound significantly increased in patients with atopic dermatitis. Conclusion:Circulating T cells in pa-tients with atopic dermatitis showed abnormal chemotactic response in vitro,which may be associated with activation state in vivo and increased serum IL-8 levels of these patients. The abnormal T cell chemotactic response may be involved in the pathogenesis of atopic dermatitis.
