RAD51B-AS1 promotes the malignant biological behavior of ovarian cancer through upregulation of RAD51B
- Author:
WEI XINYI
1
;
WANG CONGHUI
;
TANG SANGSANG
;
YANG QIAN
;
SHEN ZHANGJIN
;
ZHU JIAWEI
;
CHENG XIAODONG
;
WANG XINYU
;
XIE XING
;
XU JUNFEN
;
LU WEIGUO
Author Information
- Keywords: Ovarian cancer(OC); Long non-coding RNA(LncRNA); Metastasis; Anoikis
- From: Journal of Zhejiang University. Science. B 2024;25(7):581-593,中插4-中插16
- CountryChina
- Language:Chinese
- Abstract: Long non-coding RNAs(lncRNAs)play an indispensable role in the occurrence and development of ovarian cancer(OC).However,the potential involvement of lncRNAs in the progression of OC is largely unknown.To investigate the detailed roles and mechanisms of RAD51 homolog B-antisense 1(RAD51B-AS1),a novel lncRNA in OC,reverse transcription-quantitative polymerase chain reaction(RT-qPCR)was performed to verify the expression of RAD51B-AS1.Cellular proliferation,metastasis,and apoptosis were detected using the cell counting kit-8(CCK-8),colony-formation,transwell,and flow cytometry assays.Mouse xenograft models were established for the detection of tumorigenesis.The results revealed that RAD51B-AS1 was significantly upregulated in a highly metastatic human OC cell line and OC tissues.RAD51B-AS1 significantly increased the proliferation and metastasis of OC cells and enhanced their resistance to anoikis.Biogenetics prediction analysis revealed that the only target gene of RAD51B-AS1 was RAD51B.Subsequent gene function experiments revealed that RAD51B exerts the same biological effects as RAD51B-AS1.Rescue experiments demonstrated that the malignant biological behaviors promoted by RAD51B-AS1 overexpression were partially or completely reversed by RAD51B silencing in vitro and in vivo.Thus,RAD51B-AS1 promotes the malignant biological behaviors of OC and activates the protein kinase B(Akt)/B cell lymphoma protein-2(Bcl-2)signaling pathway,and these effects may be associated with the positive regulation of RAD51B expression.RAD51B-AS1 is expected to serve as a novel molecular biomarker for the diagnosis and prediction of poor prognosis in OC,and as a potential therapeutic target for disease management.
