DNA immunization of Mycobacterium tuberculosis resuscitation-promoting factor B elicits polyfunctional CD8+ T cell responses.
10.7774/cevr.2014.3.2.235
- Author:
Jino LEE
1
;
Jihye KIM
;
Jeewon LEE
;
Sung Jae SHIN
;
Eui Cheol SHIN
Author Information
1. Laboratory of Immunology and Infectious Diseases, Graduate School of Medical Science and Engineering, KAIST, Daejeon, Korea. ecshin@kaist.ac.kr
- Publication Type:Original Article
- Keywords:
Tuberculosis;
Resuscitation-promoting factor B;
Polyfunctional T cells;
DNA vaccines
- MeSH:
Animals;
Complement Factor B*;
Cytokines;
DNA*;
Epitopes, T-Lymphocyte;
Immunity, Humoral;
Immunization*;
Interferon-gamma;
Mice;
Mycobacterium tuberculosis*;
Peptides;
Phenotype;
Plasmids;
T-Lymphocytes;
Tuberculosis;
Vaccines, DNA
- From:Clinical and Experimental Vaccine Research
2014;3(2):235-243
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: T cell-mediated immune responses, and particularly activation of polyfunctional T cells that simultaneously produce multiple cytokines, are necessary for the control of Mycobacterium tuberculosis. In the present study, we examined if DNA immunization of Mycobacterium tuberculosis resuscitation-promoting factor B (RpfB) elicits polyfunctional T cell responses in mice. MATERIALS AND METHODS: C57BL/6 mice were immunized intramuscularly three times, at 3-week intervals, with RpfB-expressing plasmid DNA. For comparison, protein immunization was performed with recombinant RpfB in control mice. After immunization, RpfB-specific T cell responses were assessed by interferon-gamma (IFN-gamma) enzyme-linked immunosorbent spot assay and intracellular cytokine staining (ICS), and T cell polyfunctionality was assessed from the ICS data. RESULTS: RpfB DNA immunization induced not only humoral immune responses, but also CD8+ and CD4+ T cell responses. Immunodominant T-cell epitopes were identified within RpfB by assays with overlapping peptides. RpfB DNA immunization elicited a polyfunctional CD8+ T cell response that was dominated by a functional phenotype of IFN-gamma+/TNF-alpha+/IL-2-/CD107a+. CONCLUSION: RpfB DNA immunization elicits polyfunctional CD8+ T cell responses, suggesting that RpfB DNA immunization might induce protective immunity against tuberculosis.
