Lycium barbarum polysaccharides ameliorate canine acute liver injury by reducing oxidative stress, protecting mitochondrial function, and regulating metabolic pathways
- Author:
HUANG JIANJIA
1
,
2
;
BAI YUMAN
;
XIE WENTING
;
WANG RONGMEI
;
QIU WENYUE
;
ZHOU SHUILIAN
;
TANG ZHAOXIN
;
LIAO JIANZHAO
;
SU RONGSHENG
Author Information
1. College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China
2. Guangzhou General Pharmaceutical Research Institute Co.,Ltd.,Guangzhou 510240,China
- Keywords:
Acute liver injury;
Oxidative stress;
Mitochondrial dysfunction;
Metabolomics;
Lycium barbarum polysaccharides
- From:
Journal of Zhejiang University. Science. B
2023;24(2):157-171,中插10-中插21
- CountryChina
- Language:Chinese
-
Abstract:
The development of acute liver injury can result in liver cirrhosis, liver failure, and even liver cancer, yet there is currently no effective therapy for it. The purpose of this study was to investigate the protective effect and therapeutic mechanism of Lycium barbarum polysaccharides (LBPs) on acute liver injury induced by carbon tetrachloride (CCl4). To create a model of acute liver injury, experimental canines received an intraperitoneal injection of 1 mL/kg of CCl4 solution. The experimental canines in the therapy group were then fed LBPs (20 mg/kg). CCl4-induced liver structural damage, excessive fibrosis, and reduced mitochondrial density were all improved by LBPs, according to microstructure data. By suppressing Kelch-like epichlorohydrin (ECH)-associated protein 1 (Keap1), promoting the production of sequestosome 1 (SQSTM1)/p62, nuclear factor erythroid 2-related factor 2 (Nrf2), and phase Ⅱ detoxification genes and proteins downstream of Nrf2, and restoring the activity of anti-oxidant enzymes like catalase (CAT), LBPs can restore and increase the antioxidant capacity of liver. To lessen mitochondrial damage, LBPs can also enhance mitochondrial respiration, raise tissue adenosine triphosphate (ATP) levels, and reactivate the respiratory chain complexes I?V. According to serum metabolomics, the therapeutic impact of LBPs on acute liver damage is accomplished mostly by controlling the pathways to lipid metabolism. 9-Hydroxyoctadecadienoic acid (9-HODE), lysophosphatidylcholine (LysoPC/LPC), and phosphatidylethanolamine (PE) may be potential indicators of acute liver injury. This study confirmed that LBPs, an effective hepatoprotective drug, may cure acute liver injury by lowering oxidative stress, repairing mitochondrial damage, and regulating metabolic pathways.
