Insertion/deletion variants within the IGF2BP2 gene identified in reported genome-wide selective sweep analysis reveal a correlation with goat litter size
- Author:
XIN DONGYUN
1
;
BAI YANGYANG
;
BI YI
;
HE LIBANG
;
KANG YUXIN
;
PAN CHUANYING
;
ZHU HAIJING
;
CHEN HONG
;
QU LEI
;
LAN XIANYONG
Author Information
1. Lab of Animal Genome and Gene Function,College of Animal Science and Technology,Shaanxi Key Laboratory of Molecular Biology for Agriculture,Northwest A&F University,Yangling 712100,China
- Keywords:
Goat;
Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2);
Litter size;
Indel;
Marker-assisted selection (MAS)
- From:
Journal of Zhejiang University. Science. B
2021;22(9):757-766
- CountryChina
- Language:Chinese
-
Abstract:
Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2, also called IMP2) plays an essential role in the development and maturation of germ cells and embryos and is a candidate gene for goat litter size, based on a previous genome-wide selective sweep analysis. In this study, the mRNA expression level of IGF2BP2 was found to be significantly higher in a single-lamb group than in a multi-lamb group. Insertions/deletions (indels) within the goat IGF2BP2 gene, including P4-Ins-13bp and P5-Del-12bp, were verified in 918 Shaanbei White Cashmere (SBWC) female goats. The minor allelic frequencies (MAFs) of P4-Ins-13bp and P5-Del-12bp loci were 0.349 and 0.295, respectively. Analysis using the Chi-square (χ2) test showed that the genotype (χ2=14.479, P=0.006) distribution of P4-Ins-13bp was significantly different between the single-lamb and multi-lamb groups. Correlation analysis demonstrated that P4-Ins-13bp was significantly associated with goat litter size (P=0.022), and individual goats with the homozygous deletion/deletion (DD) genotype produced more litters than other goats. Therefore, considered as a potential molecular marker significantly related to lambing traits, the P4-Ins-13bp mutation of the goat IGF2BP2 gene can be used in goat breeding with practical molecular marker-assisted selection (MAS) to optimize female reproduction and improve economic efficiency in the goat industry.
