Laboratory testing and tracing analysis of a typhoid epidemic in Jiangyin city, Jiangsu Province
10.3760/cma.j.issn.0253-9624.2020.03.015
- VernacularTitle:2016年江苏省江阴市一起伤寒疫情的实验室检测及溯源分析
- Author:
Hongxia GUAN
1
;
Yong XIAO
;
Biao KAN
;
Haijian ZHOU
;
Di LUO
;
Chao SHI
;
Huimin QIAN
;
Yanhua QIAN
Author Information
1. 无锡市疾病预防控制中心微生物检验部 214023
- Keywords:
Typhoid fever;
Salmonella typhi;
Electrophoresis,gel,pulsed-field;
Tracing
- From:
Chinese Journal of Preventive Medicine
2020;54(3):323-326
- CountryChina
- Language:Chinese
-
Abstract:
To conduct outbreak identification and transmission factor analysis of typhoid epidemic occurred in Xinqiao town, Jiangyin city from June to September 2016. A total of 14 strains of Salmonella typhi isolated from confirmed cases were collected, and 65 external environment samples and 13 food samples related to the outbreak were taken. Real-time PCR was used to detect specific gene of Salmonella typhi in the samples. Conventional method was used to isolate strains. The strains isolated from both the samples and patients in the epidemic were subjected to antimicrobial susceptibility testing and PFGE molecular characteristics. Salmonella typhi strain was isolated from one external sample (well water of a deli processing plant). The results of drug susceptibility showed that 15 strains were resistant to nalidixic acid. A total of 15 strains of Salmonella typhi were divided into 2 molecular patterns by pulsed field gel electrophoresis. The fingerprints of PFGE from the 13 patients and the environmental isolate were completely consistent, and there was one band difference from the other patient isolate. Combined with the epidemiological investigation and laboratory test results, it was determined that the outbreak was caused by genetic clone of the same Salmonella typhi. Food processing plant should be one of the key links.
