Analysis on the hemagglutinin genetic characteristic of 7 measles virus isolated in Henan Province in 2017

VernacularTitle:2017年河南省7株麻疹病毒分离株血凝素基因特征分析
Author: Daxing FENG ; Lu ZHANG ; Wanyu LYU ; Jianhui YANG ; Guangwei LI ; Jin XU ; Yanyang ZHANG
Keywords: Measles virus; Hemagglutinin; Characterization; Surveillance
From: Chinese Journal of Preventive Medicine 2019;53(9):929-933
CountryChina
Language:Chinese
Abstract: Objective Analyze the genetic characteristic of Hemagglutinin(H) gene of measles viruses isolated in Henan Province in 2017. Methods Swab samples collected from 7 lab confirmed measles cases,and we got the measles virus by Vero/Slam inoculation. Fragment of H genes were amplified by reverse transcription polymerase chain reaction(RT?PCR), then the PCR products were sequenced and analyzed. Results The age of the 7 measles confirmed cases were between 1 and 50 years old,and all of them were males. All the 7 measles viruses were identified as H1a genotype,and the average distance of the nucleotides and the amino acids was 0.005, respectively. Compared with the Shanghai?191/China?vaccine, there were some changes in isolated virus,such as 240th, 397th and 381st sites in the amino acid sequence. Conclusion The measles genotype which isolated in Henan Province in 2017 was H1a. There were some difference from Shanghai?191/China?vaccine in the nucleotides sequence of H gene,which suggested that it's necessary to strengthen the monitor the variation of measles virus.