Molecular characteristics of serogroup B n eisseria meningitidis, China
10.3760/cma.j.issn.0253?9624.2019.02.006
- VernacularTitle:中国B群脑膜炎奈瑟菌分子流行病学特征分析
- Author:
Bingqing ZHU
1
;
Wanying GAO
;
Li XU
;
Yuan GAO
;
Zhujun SHAO
Author Information
1. 中国疾病预防控制中心传染病预防控制所
- Keywords:
Neisseria meningitidis,Serogroup B;
Molecular characteristic;
Vaccine antigen
- From:
Chinese Journal of Preventive Medicine
2019;53(2):153-158
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the molecular characteristics of serogroup B neisseria meningitidis in China. Methods Total of 485 (100 strains isolated from cerebrospinal fluid or blood samples of encephalomyelitis cases, and 385 strains isolated from nasopharynx of healthy carriers) Meningococcal serogroup B (MenB) strains, isolated from 29 provinces of China between 1968 and 2016, were analyzed by multilocus sequence typing (MLST) and PorA typing methods. Further, the genetic diversity of three MenB vaccine proteins, FHbp, NadA and NHBA, were analyzed. Results The 485 study strains belonged to 270 sequence types (STs), 107 of which (representing 211 strains) could be grouped into ten clonal complexes (CC). CC4821 has been the predominant lineage in China since 2005 (28.7%, n=139). The most common PorA types of MenB strains from invasive meningococcal cases were P1.5-2,2?2 (10.0%, n=10), P1.5-1,2?2 (9.0%, n=9) and P1.5-1,10?4 (9.0%, n=9). Four hundred and twenty one strains had intact fhbp gene; variant 1, 2 and 3 accounted for 12.8% (54 strains), 85.0% (358 strains) and 2.2% (9 strains) respevtively. Ten out of 432 strains (2.3%) contained complete nadA gene. All the 172 strains for which the nhba gene was sequenced had intact gene sequence which corresponded to 68 peptide types. Conclusion CC4821 was the predominant CC of MenB strains in China; the vaccine proteins were diverse about the sequences. The vaccine proteins should be carefully selected when developing MenB vaccines in China.
