Isolation and characterization of a novel strain of Natrinema containing a bop gene
- Author:
Xue-Wei XU
1
;
Min WU
;
Wei-Da HUANG
Author Information
1. 浙江大学
- Keywords:
Halophilic archaea;
Phylogeny;
Bacteriorhodopsin
- From:
Journal of Zhejiang University. Science. B
2005;6B(2):142-146
- CountryChina
- Language:Chinese
-
Abstract:
A novel member of extremely halophilic archaea, strain AJ2, was isolated from Ayakekum Lake located in Altun Mountain National Nature Reserve of Xinjiang Uygur Autonomous Region in China. The strain A J2 requires at least 10% (w/v)NaCl and grows 10% to 30% (optimum at 20%). Phylogenetic analysis based on 16S rDNA sequence comparison revealed that strain A J2 clustered to three Natrinema species with less than 97.7% sequence similarities, suggesting A J2 is a novel member of Natrinema. A bacteriorhodopsin-encoding (bop) gene was subsequently detected in the A J2 genome using the polymerase chain reaction technique. The cloning and sequencing of a 401 base pairs fragment indicated the deduced amino acid sequence of bop from A J2 is different from that reported for bacteriorhodopsins. This is the first reported detection of a bop gene in Natrinema.
