Analysis on the nuclear division index of peripheral blood lymphocytes in the 281 general population of Anhui, China
10.3760/cma.j.issn.0253-9624.2016.05.008
- VernacularTitle:安徽省281名一般人群外周血淋巴细胞核分裂指数
- Author:
Jingjing TENG
1
,
2
;
Tongjin YANG
;
Lin YE
;
Xueqing FENG
;
Yuxin ZHENG
;
Huawei DUAN
Author Information
1. 100050 北京,中国疾病预防控制中心职业卫生与中毒控制所中国疾病预防控制中心化学污染与健康安全重点实验室
2. 安徽省疾病预防控制中心毒理室
- Keywords:
Cytokinesis;
Lymphocytes;
Cytokinesis block micronucleus;
Nuclear division index;
Cytotoxity;
Cross-sectional studies
- From:
Chinese Journal of Preventive Medicine
2016;50(5):429-433
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the reference range and influeing factors of the nuclear division index (NDI) of peripheral blood lymphocyte in Chinese general population in Anhui province.Methods We selected 281 subjects from the general poulation in Anhui province,without occupational exposure to genetic toxicants and no chronic disease history.We used questionnaires to collect occupational history,age,gender,region,body mass index,smoking,and alcohol drinking status etc.NDI was measured by cytokinesis block micronucleus assay in peripheral blood lymphocytes,and the related factors were also analyzed.And NDI was used as the dependent variable,age,gender and other factors as independent variables to conduct stepwise multiple linear regression.Results We found the data of NDI according with normal distribution,and the nuclear division index was 1.71±0.22,the minimum value was 1.10 while the maximum was 2.36.The results showed that NDI value of the males (1.67±0.20) were lower than that of the females (1.76±0.24),the difference was statistically significant (t=-3.65,P＜0.001);current smokers NDI (1.66±0.18) lower than non-smokers (1.73±0.24) differences were statistically significant (t=3.06,P=0.002);the NDI of drinking groups (1.66±0.20) was lower than that of non-drinking population (1.74±0.23),the differences was statistically significant (t=3.15,P=0.002);Using multiple stepwise linear regression calibration factors and found that gender was an independent factor of NDI (β=0.098,sx =0.027,t=3.66,P＜0.001).Conclusion We set the reference value on the nuclear division index among general population of survey areas in this study,it could provide a reference for similar studies and will provide reference for better evaluation of the effects of hazards on the body.
