Genetic analysis of a child with Generalized arterial calcification of infancy due to variant of ABCC6 gene
10.3760/cma.j.cn511374-20230525-00318
- VernacularTitle:ABCC6基因变异所致婴儿泛发性动脉钙化1例患儿的遗传学分析
- Author:
Chunjuan ZHAO
1
;
Tiantian LIU
;
Fang LIU
;
Liru CUI
;
Junping WANG
Author Information
1. 首都医科大学附属北京儿童医院保定医院新生儿科，保定 071000
- Keywords:
Fever;
Generalized arterial calcification;
ABCC6 gene variant;
Whole exome sequencing;
Minigene splicing assay
- From:
Chinese Journal of Medical Genetics
2024;41(6):734-740
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the clinical manifestations and genetic basis for a rare case of Generalized arterial calcification of infancy (GACI).Methods:A 44-day-old female infant who was treated at Baoding Hospital of Beijing Children′s Hospital Affiliated to Capital Medical University on August 26, 2022 was selected as the study subject. Clinical data of the child was collected, and Trio-whole exome sequencing (Trio-WES), whole genome copy number variation sequencing (CNV-seq) and minigene splicing assay were carried out to analyze the pathogenicity of the variants.Results:The child had presented with fever and high inflammatory indicators, for which treatment with various antibiotics was ineffective. Ultrasound had revealed extensive arterial calcification and arterial wall thickening. The child was suspected for GACI with arteritis related to the primary disease. Her fever was relieved by treatment with glucocorticoid and biological agents. Trio-WES revealed that she has harbored compound heterozygous variants of the ABCC6 gene, namely c. 4404-1G>A and c. 4041+ 5G>T, for which the latter was unreported previously. Based on the guidelines from the American College of Medical Genetics and Genomics, the variants were classified as likely pathogenic (PVS1+ PM2_Supporting) and variant of unknown significance (PM2_Supporting+ PM3+ PP3), respectively. The result of CNV-seq was negative. And the minigene splicing assay has further verified that both variants can result in alternative splicing. Conclusion:For pyrexia with unknown causes and refractory to conventional treatment, it is necessary to recommend early genetic testing to avoid missed diagnosis of GACI.
