Colony Formation Assay and Chemosensitivity Test for Urologic Malignancies: A Preliminary Report.
- Author:
Jong Hoon AHN
1
;
Nam Kyu LEE
Author Information
1. Soon Chun Hyang Medical College, Seoul, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
urologic malignancy;
chemosensitivity
- MeSH:
Adenocarcinoma;
Agar;
Carcinoma, Squamous Cell;
Carcinoma, Transitional Cell;
Drug Therapy;
Humans;
Rhabdomyosarcoma;
Urinary Bladder;
Urologic Neoplasms;
Wilms Tumor
- From:Korean Journal of Urology
1989;30(3):301-306
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Use of the Hamburger-Salmon soft agar assay method for in vitro chemotherapy sensitivity testing was tried. In this assay, double soft agar was used to culture 9 human urologic tumors, including 2 renal adenocarcinomas, one Wilms' tumor, 4 bladder transitional cell carcinomas, one paratesticular rhabdomyosarcoma and one penile squamous cell carcinoma. Among these cancers, Wilms' tumor and penile carcinoma grew to the extent that they could be used in chemosensitivity testing in soft agar (> or =30 colonies per control plate). It is concluded from this study that in vitro chemosensitivity testing by colony formation assay may be a useful tool in the treatment of urologic cancers, but more experiences should be gathered.
