Protective effects of sulforaphane on the oxidative damage of kidney mitochondria complex in obese rats induced by high-fat diet
10.3760/cma.j.issn.0253-9624.2014.11.017
- VernacularTitle:莱菔硫烷对高脂诱导肥胖大鼠肾脏线粒体复合体氧化损伤的保护作用
- Author:
Hongfeng XUE
1
;
Yajie LI
;
Bing LIANG
;
Shuran WANG
Author Information
1. 150081,哈尔滨医科大学营养与食品卫生专业
- Keywords:
Mitochondrial;
Obesity;
Nephrology;
Sulforaphane;
Oxidative damage
- From:
Chinese Journal of Preventive Medicine
2014;(11):1007-1011
- CountryChina
- Language:Chinese
-
Abstract:
Objective To realize the oxidative damage of kidney mitochondrial complex in obese rats induced by high-fat diet and investigate the protective effects of sulforaphane against the damage . Methods Eighty-eight adult male SD rats were used , after 1 week adaptability feeding , 8 rats were selected as control group and given low-fat diet.The other 80 rats were given high-fat diet.After 2 weeks, the 32 diet-induced obesity models were choosen whose weight gain was higher than 40%.The 32 rats were randomly divided into 4 groups, i.e.high fat group, high fat +sulforaphane low dose group , high fat +sulforaphane middle dose group and high fat +sulforaphane high dose group.The rats in the sulforaphane low, middle and high dose groups were orally administered with sulforaphane 5, 10 and 20 mg/kg, all the 4 groups were kept feeding high-fat diet for 5 weeks.All rats were sacrificed and their kidneys were removed to assay the index of oxidative damages .Results The content of ROS (0.26 ±0.04) and MDA((0.87 ± 0.05) U/mg) in the hight-fat group were significantly higher than those in the control group ((0.20 ± 0.02),(0.57 ±0.08) U/mg) (t values were -3.02 and -4.72, P <0.05).The activity of T-AOC ((0.43 ±0.04) U/mg) and MMP (12.09 ±1.56) were lower than the control group ((0.48 ± 0.04 U/mg,(16.08 ±3.12) ) (t values were 2.06 and 2.28,P <0.05).Gavage intervention with sulforaphane , the MDA amount ( ( 0.67 ±0.05 ) , ( 0.55 ±0.05 ) , ( 0.56 ±0.07 ) U/mg ) in the sulforaphane low , middle and high dose groups were lower than the hight-fat group ( ( 0.87 ±0.05 ) U/mg (t values were 3.65,5.71 and 5.60.P<0.05).The activity of T-AOC((0.49 ±0.05),(0.55 ±0.05), (0.54 ±0.04) U/mg), T-SOD((61.07 ±2.79), (55.95 ±2.39), (60.26 ±6.02) U/mg) and the level of MMP((17.17 ±2.52), (18.24 ±2.54), (18.21 ±3.65)) were higher than in the high-fat group ((0.43 ±0.04) U/mg,(47.22 ±2.43) U/mg,(12.09 ±1.56))(tT-AOC values were -2.36, -4.83 and-4.30;tT-SOD values were -6.37, -4.71 and -5.99; tMMP values were -2.90,-3.52 and -3.50, P<0.05 ).The activity of GSH-Px in the sulforaphane low and middle dose groups ( ( 69.12 ±8.63 ) , (64.43 ±6.58) U/mg) were higher than those in the high-fat group((53.03 ±5.70) U/mg)(t values were -3.82 and -2.71,P<0.05).But there were no significant difference between the high dose group ((60.02 ±7.05) U/mg) and the high-fat group (t=-1.66,P>0.05).Conclusion High-fat diet can induce the mitochondrial oxidative dysfunction in kidney , and sulforaphane shows protective effect on the kidney mitochondrial complex from oxidative damage in obese rats induced by high -fat diet.
