Detection on drug resistance of Mycobacterium tuberculosis by microscopic observation drug susceptibility
10.3760/cma.j.issn.0253-9624.2009.01.006
- VernacularTitle:显微镜观察药物敏感性试验检测结核杆菌的耐药性
- Author:
Wen-Guo JIN
1
;
Rui-Juan ZHENG
;
Jie WANG
;
Hua YANG
;
Yuan-Sheng DING
;
You-Hui FU
;
Zhong-Yi HU
Author Information
1. 苏州大学医学院
- Keywords:
Mycobacterium tuberculosis;
Drug resistance;
Microbial sensitivity tests;
Microscopy
- From:
Chinese Journal of Preventive Medicine
2009;43(1):25-27
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of microscopic observation drug susceptibility (MODS) in detecting susceptibility of Mycobacterium tuberculosis (MTB) onto four first line anti-tuberculosis drugs.Method The 24-hole cell culture plates were used to test drug susceptibility of MTB on liquid medium,and the best detecting condition of MODS assay was probed;66 clinical isolates susceptibility to streptomycin (S),isoniazid (H),rifampin (R) and ethambatal (E) were evaluated by using MODS assay and Lowenstein-Jensen(L-J).thereafter,all the inconcordance of isolates between MODS and L-J were tested for the minimal inhibitory concentrations (MIC).Results Concordance rate of the susceptibility to S,H,R and E in 66 clinical isolates detected bv MODS and L-J was 97.0%,90.9%,95.5% and 86.4% respectively.If the results obtained by L-J were taken as a golden standard,the sensitivity,specificity,positive and negative predictive value (PPV and NPV) as well as accuracy of susceptibility test to S detected by MODS was 96.0%,97.6%,96.0%,97.6% and 97.0%;100%,85.4%,81.0%,100% and 90.9% to H;96.2%,95%,92.6%,97.4% and 95.5% to R;73.7%,91.5%,77.8%,89.6% and 86.4% to E.There were 20 inconsistent results of 16 isolates by comparing MODS with L-J.and MIC yielded 16 results of those 14 isolates showing identical results with those of the MODS,while 4 results of other 4 isolates identical with LJ.Condusion MODS method simultaneously provides drug susceptibility to S,H,R and E.MODS might be one of the rapid tools to diagnosing muhidrug-resistant tuberculosis as it is rapid,simple,inexpensive and has high concordance with L-J drug susceptibility test.
