Genotype polymorphism and its implications of mannose-binding protein allele in 5 Chinese nationalities
10.3760/j.issn:1003-9406.2001.03.010
- VernacularTitle:中国五个民族的甘露糖结合蛋白基因多态性特点及意义
- Author:
Hong SHI
1
;
Fusheng WANG
;
Lei JIN
;
Mingxu LIU
;
Weiguo HONG
;
Qingyou DU
;
Zhouyun LEI
;
Jing HOU
;
Ming SHI
;
Lihe XING
Author Information
1. 解放军第32医院
- From:
Chinese Journal of Medical Genetics
2001;18(3):202-205
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the genotypes and sequences of the exon 1 of human mannose-binding protein (MBP) allele in 5 Chinese nationalities. Methods The genotypes of MBP gene of 5 Chinese nationalities were detected by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP). The exon 1 of the MBP gene of 22 Chinese Hans was analyzed by using ABI 310 genetic analyzer. Results The DNA sequences of exon 1 of Chinese MBP gene were acquired. The allele frequencies of the codon 54 of the MBP gene (MBP-54) of 5 Chinese nationalities were 0.181(Hans), 0.128(Uygurs), 0.181(Mongols), 0.179(Tibetans) and 0.181(Yis). The allele distribution for MBP-54 mutation of 5 Chinese nationalities was in good agreement with Hardy-Weinberg equilibrium. Compared with the Hans, Uygurs had a lower MBP-54 mutation rate. There were no differences in the allele frequencies between the chronic hepatitis B patients and health controls in Chinese Hans. The mutations of the codons 52 and 57 were not detected in this study. Conclusion A higher prevalence of MBP-54 mutation was found in 5 Chinese nationalities, MBP-54 mutation was not associated with the persistence of hepatitis B.
