Comparison of denaturing high performance liquid chromatography with direct sequencing in the detection of single nucleotide polymorphism
10.3760/j.issn:1003-9406.2001.03.009
- VernacularTitle:变性高效液相色谱与直接测序法在单核苷酸多态性检测中的应用比较
- Author:
Jinxiu SHI
1
;
Shuang YANG
;
Zhengwen JIANG
;
Hongquan JIANG
;
Tianyu CHEN
;
Zhu CHEN
;
Wei HUANG
Author Information
1. Chinese Human Genome Center at Shanghai
- From:
Chinese Journal of Medical Genetics
2001;18(3):198-201
- CountryChina
- Language:Chinese
-
Abstract:
Objective Investigate the sensitivity and accuracy of the denaturing high performance liquid chromatography(DHPLC) technique for the detection of single nucleotide polymorphism(SNP). Methods Forty-one samples were detected by both DHPLC and direct sequencing. Results The comparison demonstrated that DHPLC detected all heterozygous sequences found by direct sequencing. No false-positive signals were seen in the cases of homozygous sequences. Furthermore, no false-negative results were ever obtained with heterozygous mutations or polymorphisms, or both. Conclusion DHPLC is a potent method for SNP identification especially SNP typing in large scale screening.
