Application of chromosome painting technique to analysis of structural aberration of human chromosomes
10.3760/j.issn:1003-9406.2001.02.014
- VernacularTitle:用染色体涂染技术分析五例染色体结构异常
- Author:
Huanjie YANG
1
;
Yan SONG
;
Quanzhang LIU
;
Yu LI
;
Songbin FU
;
Guiyin ZHANG
;
Pu LI
Author Information
1. Harbin Medical University
- From:
Chinese Journal of Medical Genetics
2001;18(2):135-138
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study was aimed at using chromosome painting technique to detect translocation, especially microtranslocations, on chromosomes in comparison with G-banding analysis. Methods Chromosome painting technique was applied to analysis of metaphase chromosomes of patients for detecting translocations with biotin-labeled chromosomes X, Y, 14q, 10 specific probes. Results Fluorescence in situ hybridization FISH signals were shown clearly in slides even in specimen stored at room temperature for 10 years and at -80℃. Translocations were located precisely. Conclusion Microtranslocations, which are hard to analyze by G-banding, can be detected exactly using chromosome painting technique with G-band karyotype on metaphase chromosome.
