Study on apoE gene polymorphism in Chinese endogenous hypertriglycerdemia
- VernacularTitle:中国人内源性高甘油三酯血症载脂蛋白E基因多态性的研究
- Author:
Xuemei ZHANG
1
;
Bingwen LIU
;
Huai BAI
;
Ping FAN
Author Information
1. West China University of Medical Sciences
- From:
Chinese Journal of Medical Genetics
2001;18(2):100-104
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate apolipoprotein(apo) E polymorphism and its relationship with serum lipids and apolipoproteins in Chinese patients with endogenous hypertriglyceridemia(HTG). Methods apoE genotype was assayed by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP), serum lipids were determined by enzyme method, and apolipoproteins were measured by radial immunodiffusion assay in 225 endogenous HTG patients whose fasting serum TG levels were ≥2.26 mmol/L and in 230 healthy subjects whose fasting serum lipids levels were TG<1.82 mmol/L, TC<6.21 mmol/L from a population of Chinese Han nationality in Chengdu area. Results Compared with the controls, the values of BMI,TG, TC, nHDLC, apoB100,CⅡ, CⅢ, E and TG/HDLC in HTG patients were significantly increased and the values of HDLC, apoAⅠ and apoE/apoCⅢ were significantly decreased(P<0.001). apoE3/3 genotype and allele ε3 frequency in HTG group and the control group were both the highest, and allele ε2 frequency in HTG group tended to increase than that in the control group(P>0.05). Both in HTG group and control group, the genotype of apoE2 had higher serum TG and apoE levels, lower LDLC level and decreased apoE/apoCⅢ ratio as compared with the genotype of apoE3 or apoE4(P<0.001). Conclusion Allele ε2 of apoE gene was associated with higher serum TG and apoE levels and lower serum LDLC level, and the lower ratio of apoE/apoCⅢ was associated with the higher serum level of TG in endogenous HTG.
