Study on polymorphism of D gene exons among RhD-negative Chinese Han population
10.3760/j.issn:1003-9406.2001.01.012
- VernacularTitle:中国汉族群体RhD阴性个体D基因外显子多态性研究
- Author:
Qun XU
1
;
Shixun ZHANG
;
Jianye ZHANG
;
Guiling SI
;
Yonghong SONG
;
Mei WANG
;
Jing LI
;
Wei WANG
;
Xiangmin NIE
Author Information
1. Shandong Province Blood Center
- From:
Chinese Journal of Medical Genetics
2001;18(1):39-42
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the genomic structure of 8 exons in D gene of RhD(-) Chinese Han population.Methods Polymerase chain reaction-sequence specific primers(PCR-SSP) were used to study genomic DNA from 50 samples of RhD(-) Chinese Han donors. Exons 2,3,4,5,6,7,9,10 of RHD gene and exons 1,2,4,5 of RHCE gene were specifically amplified, also intron 4 of them was amplified. Results Phenotypes of the 50 RhD(-) donors were:22 ccdee, 22 Ccdee, 3 CcdEe, 3 CcdEe. The 8 exons of RHD gene were completely absent in 25 donors with ccdee or ccdEe phenotype, while polymorphisms of D gene exons were found in 25 donors with Ccdee or CcdEe phenotype:the presence of all 8 exons of D gene in 9 donors, the absence of the 8 exons in 7 donors, the presence of exon 2 in 5 donors, the presence of exon 6 in 3 donors and the presence of exons 2,6,10 in 1 donor were demonstrated. Conclusion Polymorphisms of RHD gene exons were present among RhD(-) Chinese Han blood donors. The 8 exons of RHD gene were completely absent in donors with Rhesus cc phenotype, while 5 polymorphisms of RHD gene exons were found in donors with Rhesus Cc phenotype. The discrepancy of the RHD gene in RhD(-) individuals between Chinese Hans and Caucasians indicates that care should be exercised by clinicians in the use of the RhD genotyping results.
