Genetic polymorphism of eight STR loci in the Han population in Henan province
10.3760/j.issn:1003-9406.2001.01.011
- VernacularTitle:河南汉族群体八个STR基因座遗传多态性研究
- Author:
Bingtao HAO
1
;
Yi LI
;
Yanli YANG
;
Yingtai WANG
;
Feifei HUANG
;
Shixiu LIAO
;
Zhaocai WANG
;
Yanmei SI
;
Wenyu ZHU
Author Information
1. the People's Hospital of Henan Province
- From:
Chinese Journal of Medical Genetics
2001;18(1):35-38
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the allele frequencies of eight short tandem repeats(STR) loci:TH01,FES, D19S400,D7S820,D16S539,D20S161,D3S1545 and D5S818 in Han population in Henan province.Methods DNA was extracted with phenol-chloroform from EDTA-blood samples of the unrelated individuals in Henan province and amplified with PCR technique. The PCR product was analyzed with the undenatured PAGE vertical electrophoresis and silver-stain.Results The authors got the frequencies of the eight loci. The heterozygosities of the eight loci are 0.66, 0.67, 0.80, 0.76, 0.79, 0.79, 0.78 and 0.78; the discrimination powers are 0.83, 0.83, 0.94, 0.91, 0.93, 0.93, 0.92 and 0.92.Conclusion The heterozygosities of the eight loci are high and the frequencies are in good agreement with Hardy-Weinberg equilibrium, so the eight loci can be used in idividual identification testing.
