Malformed macrocephalous sperm with polytails and chromosomal aberrance

VernacularTitle:精子大头多尾畸形与性染色体异常
Author: Liqing FAN ¹ ; Wenbing ZHU ; Qingshan ZENG ; Yueqiu TAN ; Keli LOU ; Guangxiu LU
Author Information

1. 湖南医科大学
From: Chinese Journal of Medical Genetics 2001;18(1):28-31
CountryChina
Language:Chinese
Abstract: Objective　To investigate the relationship between sperm anomalies and chromosomal aberrance and to further understand the pathological changes of anomalous spermatozoa.Methods　Light and electronic (SEM or TEM) microscopes were used for morphological analysis. Fluorescence in situ hybridization(FISH) with chromosome X-,Y-specific probes were used for aneuploidy detection in sperms of the patient. Results　Ninety eight point seven five per cent of the Papanicolaou stained sperms were observed to be abnormal; 100% of the sperm heads were abnormal, measured under oil objective (1000×); 60.25% of the sperms had two or more tails (the maximum being eight tails). SEM and TEM demonstrated that the heads contained a large amount of cytoplasmic structure, the surfaces of heads were uneven, the nuclei were of much irregular shape; besides aberrance in quantity, anomalies occurred with centromeres, mitochondria and flagella in the tails. The results of FISH showed that the aneuploidy rate of sex chromosomes was 61.41% which was parallel to the rate of polytails.Conclusion　Even though the chromosomes in somatic cells are normal, the chromosomes in anomalous sperms may be abnormal.