Study on Circulating Tumor Cell Detection System Based on Microfluidic Chip
10.12455/j.issn.1671-7104.230595
- VernacularTitle:基于微流控芯片的循环肿瘤细胞检测系统研究
- Author:
Yuanshuai DI
1
;
Ji LUO
;
Dadi GAO
;
Haowen YANG
Author Information
1. 复旦大学附属肿瘤医院放射治疗中心,复旦大学上海医学院肿瘤学系,上海市,200032
- Keywords:
microfluidic chip;
circulating tumor cells;
sorting and enrichment;
fluorescence detection;
early screening
- From:
Chinese Journal of Medical Instrumentation
2024;48(3):277-280
- CountryChina
- Language:Chinese
-
Abstract:
Objective To achieve high throughput and high detection rate of circulating tumor cells(CTCs)in human peripheral blood,and to provide efficient and accurate early screening for cancer patients.Methods A microfluidic chip with the integration of sorting,enrichment and detection was designed,and CTCs at the single cell level were detected by fluorescence detection system to obtain the number of CTCs in samples.Results The peripheral blood samples after lysed red blood cells were used for 6 experiments.When the injection rate reached 0.2 mL/h,CTCs could reach the best detection rate of 78.6%,and the correlation coefficient within the group was above 0.8.Conclusion CTCs detection system can achieve high detection rate and has good reliability,which can provide a reliable reference for clinical research in related fields.
