Study on the Applicability of Mytomycin C as a Positive Control Material for Agar Diffusion Cytotoxicity Test
10.3969/j.issn.1671-7104.2016.03.014
- VernacularTitle:丝裂霉素C作为琼脂扩散法细胞毒性试验阳性对照材料的探讨
- Author:
Xuan JIA
1
;
Rui WANG
;
Jianfeng SHI
;
Xueying HE
Author Information
1. 北京市医疗器械检验所
- Keywords:
agar diffusion;
cytotoxicity;
Mytomycin C;
positive control material
- From:
Chinese Journal of Medical Instrumentation
2016;40(3):209-211
- CountryChina
- Language:Chinese
-
Abstract:
Objective To find a non-volatile positive material control which showed moderately or severely cytotoxic and could be obtained easily. Methods The in vitro cytotoxicity study was conducted according to the agar diffusion method in ISO 10993.5-2009 and YY/T 0127.9-2001. Each sample was placed in contacted with the surface of an agar layer overlaying a monolayer of L-929 Mouse Fibroblast cel s which were stained with neutral red vital stain at the bottom of the plate. As the sample, Mytomycin C was diluted to 2 mg/mL, 1 mg/mL and 0.5 mg/mL. Results In the group of 2 mg/mL Mytomycin C, it was observed that the cytotoxicity grade was 3. In the group of 1 mg/mL Mytomycin C, it was observed that the cytotoxicity grade was 3. In the group of 0.5 mg/mL Mytomycin C, it was observed that the cytotoxicity grade was 2. Conclusion 2 mg/mL Mytomycin C could be used as a positive material control of agar diffusion.
