Study of Collagen Sponge Extracts on Mouse Splenic Lymphocyte Rransformation in Vitro
10.3969/j.issn.1671-7104.2014.04.022
- VernacularTitle:胶原蛋白海绵浸提液对小鼠体外脾脏淋巴细胞转化作用的研究
- Author:
Shifu WU
1
;
Chenghu LIU
;
Li HOU
;
Xiaoxia SUN
;
Xiaoxiao GAI
;
Yanping SHI
Author Information
1. 山东省医疗器械产品质量检验中心 山东省医疗器械生物学评价重点实验室
- Keywords:
medical device of animal origin;
colagen sponge;
immunogen;
MTT method;
CFSE method
- From:
Chinese Journal of Medical Instrumentation
2014;(4):308-311
- CountryChina
- Language:Chinese
-
Abstract:
Immunogenicity for medical devices of animal origin is the key and diffi cult point during immune safety evaluation for these devices. This paper firstly investigated the effect of colagen sponge of animal origin on mouse splenic lymphocyte transformation and proliferation, and then analyzed the infl uence factors on the MTT method and CFSE method. The results showed that colagen sponge extract cannot signifi cantly induce transformation and proliferation of mouse splenic lymphocyte in vitro.
