Osteoporosis influence on fracture healing in ovariectomized rat: ultrastructural study
10.3760/j:issn:0529-5815.2001.03.023
- VernacularTitle:骨质疏松对卵巢切除大鼠骨折愈合影响的超微结构研究
- Author:
Xianlong ZHANG
1
;
Shuping SUI
;
Bingfang ZENG
Author Information
1. 上海市第六人民医院
- From:
Chinese Journal of Surgery
2001;39(3):241-243
- CountryChina
- Language:Chinese
-
Abstract:
Objective To elucidate the influence of osteoporosis on the fracture healing in ovariectomized rat. Methods Twenty-four female 8-month-old SD rats were randomized into two groups: sham-operated (sham) (12 rats) and bilaterally ovariectomized (OVX) (12 rats). Three months later, a femoral fracture model was ostablished in both groups. We observed the healing process by transmission elcctron microscopy(TEM) at 3, 7,14, 21, 28, and 42 days after fracture. Results TEM findings, showed that the types of fracture healing cells, their ultrastructural changes and functional state were almost identical in both groups till 21 days after fracture. In the OVX group, the calcified cartilage was not absorbed and replaced by new woven bone. A lot of dead chondrocytes were found to be embedded in a calcified chondroid matrix in 28 days after fracture. After this period, osteoclastic bone resorption was aggravated and accompanied by osteocytic osteolysis during 28－42 days of fracture healing. Conclusions Osteoporosis greatly affects the fracture healing in the later period of healing. It is demonstrated that endochondral bone formation is delayed and osteoclastic bone resorption increased which was aggravated by osteocytic osteolysis during the period of bone callus remodeling.
