Effects of dietary leucine supplementation on the hepatic mitochondrial biogenesis and energy metabolism in normal birth weight and intrauterine growth-retarded weanling piglets.
10.4162/nrp.2017.11.2.121
- Author:
Weipeng SU
1
;
Wen XU
;
Hao ZHANG
;
Zhixiong YING
;
Le ZHOU
;
Lili ZHANG
;
Tian WANG
Author Information
1. College of Animal Science and Technology, Nanjing Agricultural University, Weigang 1, Nanjing, 210095, China. tianwangnjau@163.com
- Publication Type:Original Article
- Keywords:
Leucine;
intrauterine growth retardation;
weanling piglets;
mitochondrial biogenesis;
energy metabolism
- MeSH:
Adenosine Triphosphate;
Birth Weight*;
Citrate (si)-Synthase;
Diet;
DNA, Mitochondrial;
Energy Metabolism*;
Fetal Growth Retardation;
Leucine*;
Liver;
Malate Dehydrogenase;
Nuclear Respiratory Factor 1;
Organelle Biogenesis*;
Oxidoreductases;
Parturition*;
Peroxisomes;
Pyruvic Acid;
Research Design;
RNA, Messenger;
Sirtuin 1;
Transcription Factors
- From:Nutrition Research and Practice
2017;11(2):121-129
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND/OBJECTIVES: The study was conducted to evaluate the effects of dietary leucine supplementation on mitochondrial biogenesis and energy metabolism in the liver of normal birth weight (NBW) and intrauterine growth-retarded (IUGR) weanling piglets. MATERIALS/METHODS: A total of sixteen pairs of NBW and IUGR piglets from sixteen sows were selected according to their birth weight. At postnatal day 14, all piglets were weaned and fed either a control diet or a leucine-supplemented diet for 21 d. Thereafter, a 2 × 2 factorial experimental design was used. Each treatment consisted of eight replications with one piglet per replication. RESULTS: Compared with NBW piglets, IUGR piglets had a decreased (P < 0.05) hepatic adenosine triphosphate (ATP) content. Also, IUGR piglets exhibited reductions (P < 0.05) in the activities of hepatic mitochondrial pyruvate dehydrogenase (PDH), citrate synthase (CS), α-ketoglutarate dehydrogenase (α-KGDH), malate dehydrogenase (MDH), and complexes I and V, along with decreases (P < 0.05) in the concentration of mitochondrial DNA (mtDNA) and the protein expression of hepatic peroxisome proliferator-activated receptor-γ coactivator 1α (PGC-1α). Dietary leucine supplementation increased (P < 0.05) the content of ATP, and the activities of CS, α-KGDH, MDH, and complex V in the liver of piglets. Furthermore, compared to those fed a control diet, piglets given a leucine-supplemented diet exhibited increases (P < 0.05) in the mtDNA content and in the mRNA expressions of sirtuin 1, PGC-1α, nuclear respiratory factor 1, mitochondrial transcription factor A, and ATP synthase, H+ transporting, mitochondrial F1 complex, β polypeptide in liver. CONCLUSIONS: Dietary leucine supplementation may exert beneficial effects on mitochondrial biogenesis and energy metabolism in NBW and IUGR weanling piglets.
