Adiponectin receptor mediated berberine to alleviate renal lipid toxicity in mice with diabetic kidney disease
10.12025/j.issn.1008-6358.2024.20231508
- VernacularTitle:脂联素受体介导小檗碱减轻糖尿病肾病肾脏脂毒性
- Author:
Yueyue WU
1
;
Zaoping CHEN
;
Li SHENG
;
Xinmei HUANG
;
Rui ZHANG
;
Mengxue YANG
;
Jun LIU
Author Information
1. 复旦大学附属上海市第五人民医院内分泌代谢科,上海 200240
- Keywords:
adiponectin receptor;
berberine;
diabetic kidney disease;
renal lipid toxicity;
db/db mice
- From:
Chinese Journal of Clinical Medicine
2024;31(3):433-439
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore whether adiponectin receptor 1/2(AdipoR1/R2)mediates berberine to alleviate renal lipid toxicity in mice with diabetic kidney disease.Methods C57BLKS/J db/db mice and db/m mice were selected and divided into 4 groups,with 8 in each group.db/db mice and db/m mice were given berberine(150 mg·kg-1·d-)or placebo by gavage for 12 weeks,respectively.After intervention,blood glucose,blood lipids,urinary microalbumin/creatinine ratio and urinary albumin excretion rate were determined.Kidney tissues were taken for pathological examination,and oil red O staining,immunohistochemistry,ELISA and Western blotting were used to examine lipid deposition,AdipoRl/R2 expression and fibrosis markers expressions,etc.,respectively.The human glomerular endothelial cell line(HGEC)was used to verify the role of AdipoR1/R2 in berberine alleviating renal lipid toxicity.Results After berberine intervention,the body weight,blood glucose,lipids,proteinuria,renal lipid deposition,and renal pathological changes decreased in db/db mice(P＜0.05);the expressions of AdipoR1 and AdipoR2 increased,expressions of TGF-β1 and Col-Ⅳ decreased,and the expressions of PPAR-α and p-AMPK increased in kidney tissue of db/db mice(P＜0.05).In high-fat cultured HGEC cells treated with berberine-treated db/db mouse serum,the expressions of TGF-β1 and Col-Ⅳ decreased,while the expressions of PPAR-α and p-AMPK increased(P＜0.05);after AdipoR1/R2 on HGEC cells were silenced,the expressions of TGF-β1 and Col-Ⅳ increased,while the expressions of PPAR-α and p-AMPK decreased(P＜0.05).Conclusions Berberine can reduce renal lipid toxicity in diabetic nephropathy mice,and adiponectin receptor may mediate this effect of berberine.
