GO-CoA-Tat improves lipid metabolism and oxidative stress in mice with fatty liver disease
10.12025/j.issn.1008-6358.2023.20231029
- VernacularTitle:GO-CoA-Tat改善脂肪肝小鼠脂质代谢和氧化应激
- Author:
Hai-Yan WANG
1
;
Yu-Lin LI
;
Jing-Xian HU
;
Hao ZHOU
;
Miao JIANG
;
Shao-Ren ZHANG
Author Information
1. 复旦大学附属金山医院消化内科,上海 201508
- Keywords:
high-fat diet;
non-alcoholic fatty liver disease;
GO-CoA-Tat;
ghrelin O-acyltransferase;
oxidative stress
- From:
Chinese Journal of Clinical Medicine
2023;30(6):965-970
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of GO-CoA-Tat,an inhibitor of ghrelin O-acyltransferase(GOAT),on lipid metabolism and oxidative stress in mice with non-alcoholic fatty liver disease(NAFLD)induced by high-fat diet(HFD).Methods Twenty-four C57BL/6 male mice were selected and divided into control group,HFD group and GO-CoA-Tat group,with 8 mice in each group.The mice in control group was given standard diet,the mice in HFD group and GO-CoA-Tat group were given HFD,and the mice in GO-CoA-Tat group was given daily intraperitoneal injection of GO-CoA-Tat from 3rd week of feeding.Food intake and body mass of mice were measured weekly.After 8 weeks,serum and liver samples were collected,liver weight was measured,and fat droplets were detected by hepatocyte oil red O staining;biochemical indexes such as serum triglyceride(TG),total cholesterol(TC),alanine aminotransferase(ALT),aspartate aminotransferase(AST)and oxidative stress indexes of liver such as glutathione(GSH),superoxide dismutase(SOD)and malondialdehyde(MDA)were measured.Results Hepatic steatosis was observed after feeding with HFD for 8 weeks,which was significantly relieved in GO-CoA-Tat group compared with HFD group.Compared with HFD group,the food intake,body weight and liver weight of mice in GO-CoA-Tat group decreased(P＜0.05).Compared with HFD group,the content of TG in liver of mice in GO-CoA-Tat group decreased,the concentrations of serum TG and TC decreased(P＜0.05),and the concentrations of liver GSH and SOD increased(P＜0.01),liver MDA decreased(P=0.005),and the serum ALT and AST decreased(P＜0.05).Conclusion GO-CoA-Tat can improve lipid metabolism and oxidative stress in the liver of NAFLD mice,thus play a protective role in the liver.
