Impact of burn injury on choke vessels of skin of rats
10.3760/cma.j.cn114453-20220405-00099
- VernacularTitle:热损伤对大鼠皮肤choke区血管的影响
- Author:
Xueying CHEN
1
;
Li TENG
;
Mengqing ZANG
Author Information
1. 中国医学科学院北京协和医学院整形外科医院瘢痕综合治疗中心，北京 100144
- Keywords:
Cicatrix;
Thermal injury;
Choke vessel;
Three-perforasome perforator flap;
Skin blood perfusion;
Scar flap
- From:
Chinese Journal of Plastic Surgery
2022;38(6):680-690
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a standardized burn injury in rat model and to explore effect of the burn injury to choke vessel of dorsal skin flap in the rat model.Methods:114 male Sprague Dawley rats were randomly divided into 3 groups, 38 rats of each group. The dorsal three-perforasome perforator flap of rats in different groups was contacted with 100 ℃ water for 10, 15 or 20 seconds, respectively. Blood perfusion of the injured area was monitored for 7 days using PeriCam PSI system. Lead oxide-gelatine angiogram was performed to observe distribution of skin vessels. Histological analysis was performed to observe depth of burn injury and quantify the microvascular density and diameter. The measurement datas were expressed as Mean±SD, and statistical analysis was performed by t-test, one-way ANOVA and resuable ANOVA. Results:10, 15, 20-s scald resulted in a superficial second-degree burns, a deep second-degree burns, and deep third-degree burns, respectively. At day 7 after burn injury, perfusion was comparable with the pre-burn level in the 10 s-scalding group[speckle flow index (SFI): 143.25±30.40 vs. 140.28±26.35, P=0.828], was slightly lower than pre-burn level in the 15 s-scalding group (106.20±10.30 vs. 119.31±9.66, P=0.072), and significantly decreased in the 20 s-scalding group (67.49±19.93 vs. 136.37±18.96, P=0.001). The density of the blood vessels in the entire three-perforasome area recovered to the pre-burn level within 14 days in the 10 s-scalding group, slightly increased in the 15 s-scalding group, and obviously decreased in the 20 s-scalding group. At day 7 after burn injury, the microvascular density in the choke vessel zone was equal to the pre-burn level in the 10 s-scalding group [(29.16±2.38)/mm 2 vs. (27.74±3.66)/mm 2, P=0.696] and 20 s-scalding group [(30.80±2.27)/mm 2 vs. (27.74±3.66)/mm 2, P=0.407], and significantly increased in the 15 s-scalding group [(36.68±4.65)/mm 2 vs. (27.74±3.66)/mm 2, P=0.027]. At day 7 after burn injury, the microvascular diameter of choke vessels was equal to the pre-burn level in the 10 s-scalding group [(35.61±2.49) μm vs. (41.74±3.31) μm, P=0.938] and 15 s-scalding group [(52.88±4.97) μm vs. (41.74±3.31) μm, P=0.058], and was significantly decreased in the 20 s-scalding group [(37.57±5.33) μm vs. (41.74±3.31) μm, P=0.001]. Conclusions:The superficial second-degree burn injury don’t impact the choke vessels. The deep second-degree burn injury increased the microvascular density in the choke vessel zone and the post-burn blood perfusion can recover to the pre-burn level. The deep third-degree burn injury damage the blood vessels in the dorsal skin and the perfusion can not restore.
