Effect of silencing syndecan-1 on the migration, invasion and angiogenesis abilities of human umbilical vein endothelial cells
10.3760/cma.j.cn114453-20210731-00326
- VernacularTitle:沉默多配体蛋白聚糖-1表达对人脐静脉内皮细胞迁移、侵袭及血管生成能力的影响
- Author:
Dexin ZHANG
1
;
Junjie LIN
;
Junbo QIAO
;
Junhua LI
;
Haoran ZHANG
;
Wenqiu WANG
;
Yujiao WANG
Author Information
1. 郑州大学第三附属医院血管瘤外科 河南省血管瘤与血管畸形医学重点实验室，郑州 450003
- Keywords:
Vascular malformations;
Syndecan-1;
Human umbilical vein endothelial cells (HUVECs);
Migration;
Invasion;
Angiogenesis
- From:
Chinese Journal of Plastic Surgery
2022;38(6):691-697
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of syndecan-1 (SDC1) in human venous malformations and the effect of SDC1 silencing on the migration, invasion and angiogenesis abilities of human umbilical vein endothelial cells (HUVECs).Methods:Twenty surgically resected samples of venous malformation were collected from 20 patients with hemangioma at the Third Affiliated Hospital of Zhengzhou University from December 2020 to June 2021 (10 males and 10 females, aged 1-57 years, median age 7 years). HUVECs were purchased from American Type Culture Collection (ATCC). Immunohistochemistry staining was used to detect the expression of SDC1 in venous malformations. HUVECs cultured in vitro were divided into two groups, and were transfected with SDC1 small interfering RNA (siRNA) (si-SDC1 group) or control siRNA (control group), respectively. The siRNA with the best silencing effect was screened and tested by real-time quantitative PCR and Western blotting for follow-up experiments. The migration, invasion and angiogenesis abilities of HUVECs were measured by scratch test, Transwell migration and invasion test, and angiogenesis test, respectively. Results:(1) SDC1 was positive in 13(65.0%) of the 20 venous malformations and was mainly expressed in the cytoplasm of venous endothelial cells. (2) Compared with HUVECs in the control group, the expression of SDC1 mRNA and protein in HUVECs cells in si-SDC1 group both decreased ( P<0.05), indicating the synthesized siRNA targeting SDC1 could silence the expression of SDC1. (3) The migration, invasion and angiogenesis abilities of HUVECs were enhanced in si-SDC1 group( P<0.05). Conclusions:SDC1 was expressed in most venous malformations. After silencing SDC1, the migration, invasion and tube formation abilities of HUVECs were enhanced.
