The synergistic effect of raltitrexed combined with X-ray radiation on human laryngeal cancer Hep-2 cells
10.12025/j.issn.1008-6358.2017.20170522
- VernacularTitle:雷替曲塞联合X线照射对人喉癌细胞Hep-2的协同作用
- Author:
Shu WANG
1
;
Xuan LI
;
Tian-Kui QIAO
Author Information
1. 复旦大学附属金山医院肿瘤科
- Keywords:
raltitrexed;
X-ray;
Hep-2 cells;
apoptosis;
cell cycle
- From:
Chinese Journal of Clinical Medicine
2017;24(6):879-883
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the synergistic effect of raltitrexed combined with radiotherapy on Hep-2 cells and its potential mechanism.Methods:CCK-8 assay was used to determine the cell viability after Hep-2 cells were treated with different concentrations of raltitrexed.Hep-2 cells were randomly divided into the control group,the raltitrexed group,the radiation group,and raltitrexed + radiation group.Cell survival curves were fitted with a single-hit multi-target model,cell viability and sensitization enhancement ratio(SNR) of the 4 groups were detected,and apoptosis and cycle changes of Hep-2 cells in 4 groups were detected by flow cytometry.Results:CCK-8 assay proved that raltitrexed could suppress cell viability in a dose-dependent manner.The single-hit multi-target model showed that the SER of mean death dose (D0) was 1.272.Furthermore,the apoptosis rate and the number of Hep-2 cells at G2/M phase were significantly increased in the group treated with the combination of raltitrexed and X-ray as compared to the group treated with X-ray alone (P＜0.05).Conclusions:The inhibitory effect of raltitrexed combined with X-ray on human laryngeal cancer Hep-2 cells may be related to the induction of tumor cell apoptosis and the increase of G2/M phase arrest.
