The role and mechanisms of NCX1 on proliferation and invasion of hepatocellular carcinoma
10.12025/j.issn.1008-6358.2017.20170291
- VernacularTitle:钠钙交换器1对肝细胞肝癌增殖与侵袭的影响
- Author:
Hui TIAN
1
;
Bi-Wei YANG
;
Zhi-Ying ZHAO
;
Pei-Xin HUANG
;
Wen-Qing TANG
;
Ting-Ting FANG
;
Jing-Lin XIA
Author Information
1. 复旦大学附属中山医院老年病科
- Keywords:
Na+/Ca2+ exchanger 1;
hepatocellular carcinoma;
invasion;
proliferation;
epithelial-mesenchymal transition
- From:
Chinese Journal of Clinical Medicine
2017;24(6):873-878
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role and molecular mechanism of Na+/Ca2+ exchanger 1 (NCX1) in the invasion and proliferation of hepatocellular carcinoma (HCC),and to provide new therapeutic targets for the clinical diagnosis and treatment of hepatocellular carcinoma.Methods:Quantitative real-time PCR (qRT-PCR) and Western blotting were used to detect NCX1 mRNA and protein expression in different HCC cell lines.Lentiviral vectors pGC-SIL-GFP-shRNA-NCX1 and pGC-FU-GFP-NCX1 were constructed and transfected into hepatoma cells.Cell migration and invasion experiments were used to observe the effect of NCX1 on the migration and invasion of HCC cells;cell proliferation assay was used to observe the effect of NCX1 on the proliferation of hepatoma cells;Western blotting and ELISA were used to detect the expressions of NCX1-related proteins.Results:The expression of NCX1 in high metastatic potential HCC cell lines (MHCC97H,HCCLM3) was higher than that of low metastatic potential HCC cell lines (HepG2,Huh7,SMMC-7721),and the difference was statistically significant (P＜0.05).After transfection of NCX1,the invasion and proliferation ability of HCC cells increased significantly (P＜0.05);the secretion of cytokines (TGF-β1,IL-6 and TNF-α) increased significantly (P＜0.05);the expressions of epithelial-mesenchymal transition (EMT) related proteins in HCC cells were significantly increased (P＜0.05).Conclusions:NCX1 can promote the growth,invasion and metastasis of hepatocellular carcinoma by increasing the expressions of EMT-related proteins in HCC cells,and it is a potential target for the diagnosis and treatment of liver cancer.
